FastQCFastQC Report
Wed 25 May 2016
SRR1779605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209871
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT157367.4979392102767894No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG22351.0649398916477264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC11630.5541499301952152No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT10450.4979249157815992No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT6680.31829075956182606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6280.2992314326419563No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2970.14151550238003346No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2680.12769749036312783No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2570.12245617546016363RNA PCR Primer, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT150.002223534569.990711
GATAAAC207.918751E-569.9907133
TATCTTC150.002223534569.9907116
AGGGGGG17850.065.2854570
AGTTAAA550.063.62792232
GTATGAC1500.063.03669442
GTTAAAC453.8380676E-1062.21395533
TTATGCT401.0586518E-861.27106552
TAGTATG352.962006E-760.00633240
CTTTACA352.966899E-759.992041
GTCTTAT650.059.25114449
TGCTAGA950.058.95359455
CGTCTTA601.8189894E-1258.3673148
CTAAAAC1200.058.325591
TTTATAC308.362922E-658.325593
CGTATGA1650.057.29242741
GCTAGAA1100.057.27877456
GTAAATC553.6379788E-1157.2651333
AGATAAA252.3880863E-455.9925732
TTAAACT509.767973E-1055.9925734