FastQCFastQC Report
Wed 25 May 2016
SRR1779604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329018
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233647.101131245099053No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTGC252.3887007E-455.99914635
ATTCCCA200.00694389152.50718359
GTATTAC200.006948066452.4992031
ACCGTAA200.006948066452.49920337
ATCATTA503.470026E-642.0057459
GTATAAG459.188408E-538.8882981
AAGTACA450.004301155531.11536862
GTAGCCT450.004304356431.11063839
TCAATAC450.004304356431.1106383
ACACTCC450.004304356431.11063819
CATTAAA802.4589172E-630.6291961
TATCATT703.480101E-530.00410558
TCATTAA703.480101E-530.00410560
GATGTAT703.4837147E-529.99954438
GTACTGG604.977098E-429.1662221
TATAAGA604.977098E-429.1662222
GTATGCC500.00720354427.99957348
TACTGCG500.00720354427.9995737
CGCCATA657.9479045E-426.92266853
CGTATCA808.653119E-526.24960156