FastQCFastQC Report
Wed 25 May 2016
SRR1779604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329018
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT136004.133512452206262No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG19300.5865940465263298No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC10020.304542608611079No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT9140.27779635156739146No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6130.18631199508841462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3970.12066209143572693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG14850.063.1585470
TGATAAG700.054.9860330
GCTAATA200.00692124852.55055643
TAGCACT200.006954632252.4866644
CACAGGT200.006954632252.48666415
TCGTATG28000.052.11176340
TGAAAAA28950.052.0938459
CGTATGC27250.051.779841
TTGAAAA27750.051.32029358
GTATGCC27400.051.27195442
CTCGTAT28050.051.27039339
CTTGAAA27350.051.17529757
CCGTCTT26100.051.14893747
ATGAAAA1850.051.06810858
GAAAAAA31300.050.97746360
TATGCCG27750.050.7515343
TGCTTGA26900.050.63130655
GTCTTCT27000.050.6042449
TCTGCTT26350.050.52298753
TCTCGTA28650.050.44093738
Produced by FastQC (version 0.11.5)