FastQCFastQC Report
Wed 25 May 2016
SRR1779603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371221
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180844.87149164513861No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGG450.004304739631.11111329
GCATAAG604.9778714E-429.1666661
ATCTAAG500.007204179728.0000021
TTACTGA500.007204179728.00000250
TCGTAAC500.007204179728.00000220
TCCATAC700.001224990425.064
AGCACTT1001.3954475E-524.5000025
CCAGTAT750.001830704723.33333444
CCAGTAC750.001830704723.3333343
TCTTGTG800.002663779821.8752
CACTTTG800.002663779821.8757
GTATTGA850.003786003420.5882341
AACCCGC850.003786003420.58823419
ATAATGC850.003786003420.58823425
ACCCGAT850.003786003420.5882347
CCCATAG850.003786003420.5882341
TCTTAGG1055.45699E-420.02
GGACCAT900.005270268319.4444456
GATAGAC900.005270268319.44444535
AGCTCCC1107.464089E-419.09090810
Produced by FastQC (version 0.11.5)