FastQCFastQC Report
Wed 25 May 2016
SRR1779603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371221
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT84422.2741170354047857No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG10880.29308686739166157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5500.14815972156747598No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT4060.10936881264799135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT401.0653821E-861.24304639
CTTAAGC750.060.6679551
CGTCTTA2250.056.0616148
AGGGGGG9100.053.4482770
TCGTATG16250.052.76323740
TAATCCG200.00695517352.486977
CTCGTAT16700.051.97014639
GTATGCC16300.051.59044342
CGTATGC15300.051.5133241
TCTCGTA17450.051.34087438
TATGCCG16350.050.78976443
CCGTCTT12650.050.6881247
TCTTAAG1050.050.0010650
ATCTAGT1750.049.99432837
CTTGAAA15450.048.9528657
GTCTTAA1150.048.69668649
ATCTCGT18350.048.6320837
TGAAAAA21350.048.53869659
GTATTCT651.8553692E-1048.46256349
CGTATGA1450.048.31594541
Produced by FastQC (version 0.11.5)