FastQCFastQC Report
Wed 25 May 2016
SRR1779602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552721
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222514.025720028730589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACA305.875168E-446.66924
ATCATTA1800.035.001959
CGTATCA1850.034.05282256
GCCGTAT1950.032.30652654
CCCTGTG450.00430540231.11287
TTGGATA450.004307308731.10998556
CATTAAA2150.030.93191361
TCATTAA2150.029.30391960
GCTATAC604.982041E-429.1656111
TATCATT2200.028.63791858
GTATCAT2250.028.00151857
GTTTAGA500.00720845627.9989852
GTTAGAG657.95576E-426.9221043
CCGTATC2350.026.8075455
TAGATCT2400.026.2490539
TCTACCC1101.0156255E-625.4536253
CTACCCA901.9264016E-423.33464
CTATCGA750.001832217923.3324891
ATTAAAA3450.022.32005162
TAGAGAG15900.022.01377370
Produced by FastQC (version 0.11.5)