FastQCFastQC Report
Wed 25 May 2016
SRR1779602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552721
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT123882.2412754355271467No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG17750.3211385129206236No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC13090.23682834558484298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7500.1356923294030804No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT7260.13135017486218184No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT5530.10005047754653795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG12800.059.60544670
CGTCTTA1500.058.3611748
TAACTCG553.6379788E-1157.2637736
TTGAAAA22000.053.45585658
TCGTATG23550.052.91479540
CTTGAAA21500.052.9130657
CTCGTAT23300.052.43092739
CGTATGC22550.052.31673441
GTATGCC22500.052.2868742
CCGTCTT20150.052.13404547
TATGCCG22650.051.63143543
TGAAAAA25900.051.6228959
GCTTGAA21800.051.54262556
TCTTCTG19650.050.6093850
GAAAAAA27600.050.3454460
TCTCGTA24950.049.9365938
ATTATCT25400.049.8740934
TTATCTC24950.049.79181735
TTCTGCT19150.049.54917552
GTATTCT850.049.43534549
Produced by FastQC (version 0.11.5)