FastQCFastQC Report
Wed 25 May 2016
SRR1779601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206459
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205859.970502617953201No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2420.11721455591667111No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAT150.00222231370.04
TTCACTA150.00222231370.057
CACTAAG150.00222231370.059
AGCCTTT150.00222231370.068
CCTGATA150.00222231370.032
GTAAACG150.00222231370.01
GAAGCTT252.3864294E-455.99999638
TATGATG200.00694400152.5000045
AAACGTG200.00694400152.5000045
GTGGGTT305.8681413E-446.6666644
GGGGACT350.001253377940.036
AATTCCG350.001253377940.046
TTTCTGT350.001253377940.039
CGTATCA459.175692E-538.8888956
TCATTAA459.175692E-538.8888960
TGGGTTC400.00241485435.0000045
CATAGAC400.00241485435.0000043
GTGTATA400.00241485435.0000041
GGACCAT400.00241485435.0000041
AGGGATT400.00241485435.00000431
Produced by FastQC (version 0.11.5)