FastQCFastQC Report
Wed 25 May 2016
SRR1779601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206459
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT116575.646157348432376No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG17320.8389074828416295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC12870.6233683201022964No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6600.3196760615909212No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4820.2334603964951879No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.20197714800517294No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT2170.10510561418974228No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC150.002223563469.990072
TTTATAC301.0211261E-769.990073
CACACTC150.002223563469.990075
TATCGGA150.002223563469.9900764
ATAGCAA501.4551915E-1162.9910732
TAGCAAC453.8380676E-1062.213433
AGGGGGG11650.061.57924770
TATACAA252.3881023E-455.992063
CTTTTAC200.006934594452.5179952
TCTACTG407.4365744E-752.5179950
TCTTTTA200.006934594452.5179951
CTTAATA200.00694789852.4925541
ACCATTC200.00694789852.49255421
TAGAAAC200.00694789852.49255433
ATAGAAA200.00694789852.49255432
TTATCTG200.00694789852.49255419
CATTCCC200.00694789852.49255423
CTAAAAC407.4614945E-752.4925541
CTCGTAT22850.050.5521239
TCTCGTA23150.050.50182738