FastQCFastQC Report
Wed 25 May 2016
SRR1779600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533209
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265914.986975088567522No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG400.002418909734.9981231
ATCATTA1450.033.79446459
GTATCAT1350.033.7050657
CGTATCA1400.032.4982656
ATATGCG604.9795787E-429.167844
TCATTAA1800.027.22331860
CCTTGGA1101.0156673E-625.453182
CTTAGAG700.001226075524.9986612
TAGAGAG12000.023.33427270
ATATGCT750.001831319423.3342724
TAGATCT1953.092282E-1123.33427239
TAAGCCG750.001831319423.3342725
CATTAAA2250.023.3342761
GTATGAG750.00183231923.3320851
TATCATT2056.002665E-1122.19601458
GTCTGTT800.002663217321.87793230
CCGTATC2259.094947E-1221.77661155
ATACAAC1304.3397085E-621.5373063
ATTAAAA2151.1459633E-1021.16364162
GAGTAGG1506.3754123E-720.9988751
Produced by FastQC (version 0.11.5)