FastQCFastQC Report
Wed 25 May 2016
SRR1779600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533209
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT149732.8080921364793165No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG22250.4172847795142242No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC12300.23067877698988576No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT9810.18398039042851866No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT7010.13146814851212188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5800.10877535825539329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15600.058.7685170
GTGTACA200.00695633952.4879041
GTATGCC26750.049.75482642
TCGTATG28000.049.37288340
CGTATGC27250.049.34675641
CTCGTAT28000.048.87290239
TATGCCG27350.048.5352543
TCTCGTA28850.048.0395338
CCGTCTT24700.047.65398847
TTGAAAA28400.047.20753558
TGAAAAA29900.046.71244459
GCCGTCT25350.046.70847746
GAAAAAA31750.046.4118160
GTCTTCT25200.046.4043149
TCTTCTG25550.046.04269850
TTCTGCT25300.045.9398152
AATCTCG30450.045.73658436
GCTTGAA28400.045.48193456
TCTGCTT26250.045.348453
ATCTCGT30700.045.13617737
Produced by FastQC (version 0.11.5)