FastQCFastQC Report
Wed 25 May 2016
SRR1779599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488058
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295246.049281028074533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC400.002418073835.00000432
ATTCGGT500.007206369727.99999818
TGAATCG500.007206369727.99999854
CGTATCA959.383393E-625.78947456
ATACAAT700.001225543625.06
AAGTATC750.001831526923.33333445
TACCCAG952.7782307E-422.1052655
TACGACG800.002664969321.8750024
TACAATG1154.1132684E-521.3043487
TAGAGAG9950.021.10552870
CCCTTAA1003.9291597E-421.01
ATAAGAG1356.012964E-620.740743
GTCTACT850.003787686620.5882361
GTGTATG1205.7090598E-520.4166681
AGTATCT1055.460371E-420.00000246
AACCGCG1257.8145924E-519.67
GTATAAG1257.8145924E-519.61
CATAGTC900.005272601319.44444355
GCCGTAT900.005272601319.44444354
ACAATGC1301.0560525E-418.8461538
Produced by FastQC (version 0.11.5)