FastQCFastQC Report
Wed 25 May 2016
SRR1779599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488058
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT163433.348577423175114TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG21440.4392920513545521TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC12870.26369816702113275RNA PCR Primer, Index 5 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT8690.1780526085014486TruSeq Adapter, Index 5 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT6230.1276487630568498RNA PCR Primer, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5800.11883833478807847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16450.057.23094670
GATCGTA252.390309E-455.99696739
GCCGTAT900.054.4805746
TATGCCG28000.054.0302943
CGTATGC29300.053.65389641
ATCTCGT28900.053.64211737
GTATGCC28500.053.568442
TCGTATG29350.053.54054340
CTCGTAT29100.053.39916639
TCTCGTA29500.053.1442238
GTCTTCT24400.052.9545949
CCGTCTT24950.052.92086447
CTTGAAA28200.052.8694857
ACGTAAT200.006931444652.53483647
TCGTATT407.478193E-752.49715440
ATGCCGT28950.052.1363144
CGTCTTC25300.052.05032748
TTGAAAA29800.051.32272358
TTCTGCT24400.051.2324952
GCTTGAA28450.051.1852256