FastQCFastQC Report
Wed 25 May 2016
SRR1779598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182904
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1842210.071950312732362No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG13190.7211433320211695No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2220.12137514761842277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT252.3860292E-455.99846665
TAGAGCG305.867165E-446.6653927
GGGATCC350.001251486740.0098464
ATCTCGG2500.037.79896542
ATCATTA2500.037.79896559
TCGGTGG2800.037.49897445
CGTATCA2450.037.1418456
GTATCAT2650.036.9801257
GCCGTAT2350.035.74370654
TCTCGGT2450.035.7133143
TAGATCT2850.035.6130639
TCATTAA2800.034.99904360
CCGTATC2400.034.99904355
CGGTGGT2800.034.99904346
TGTAGAT3000.034.99904337
CGCCGTA2500.034.99904353
AGATCTC2750.034.36269840
CATTAAA3000.033.8324161
GTAGATC3000.033.8324138
CTCGGTG2750.033.09000444