FastQCFastQC Report
Wed 25 May 2016
SRR1779598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182904
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT88464.83641691816472No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT29911.6352840834536149No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22201.2137514761842276No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG15560.8507195031273236No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6180.3378821676945283No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2760.15089883217425534No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2630.14379127848488824No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT2480.135590254997157No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2140.11700126842496611No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT1990.10880024493723484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG150.002223834669.984694
ATTATAC13200.062.2969741
TTCGTAA401.0622898E-861.23660730
AGGGGGG10050.060.58376370
CGTCTTA308.268438E-658.43239248
CGTATGA1150.054.78560341
TTGCGAT200.00690376752.57475746
AAACTCG407.4607306E-752.48851836
CTGTATA200.006948747752.48851839
ACTGATC200.006948747752.4885188
TAACGTA200.006948747752.48851838
TGTAATA200.006948747752.48851824
TCGGTAA200.006948747752.48851824
TCGTAAA608.367351E-1152.48851431
TCGTATG21150.052.1162640
CTCGTAT20250.052.0133139
AATCTCG20050.052.0085736
CCGTCTT18500.051.9258747
GTCAATC19850.051.82745733
CGTCTTC19150.051.81107348
Produced by FastQC (version 0.11.5)