FastQCFastQC Report
Wed 25 May 2016
SRR1779597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42467
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG767918.082275649327713No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG5871.3822497468622696No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1140.2684437327807474No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530.12480278804718958No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460.10831940094661738No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC450.10596463136082135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAA150.002210133270.010
ATCAGAA150.002210133270.012
ACTAAAA150.002210133270.012
CGTCTGG200.00690623452.5000049
CTAAAAA200.00690623452.50000413
TACTAAA200.00690623452.50000411
TCTGGCG407.313465E-752.50000411
TCTCGGT800.048.12500443
GTCTGGC305.8153004E-446.66666410
ATCTCGG851.8189894E-1245.29411742
TAGATCT851.8189894E-1245.29411739
TGTAGAT851.8189894E-1245.29411737
TATCATT703.6561687E-1045.058
CATTAAA802.7284841E-1143.75000461
CCGTATC802.7284841E-1143.75000455
TCGGTGG1050.043.33333645
AGATCTC901.8189894E-1242.77777540
GTAGATC901.8189894E-1242.77777538
GTGTAGA901.8189894E-1242.77777536
GATCTCG901.8189894E-1242.77777541