FastQCFastQC Report
Wed 25 May 2016
SRR1779597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42467
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT36828.670261614900982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10522.4772176042574237No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG4391.033743848164457No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3190.7511714978689336No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2110.4968563826029623No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1940.4568252996444298No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT1340.31553912449666804No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT1080.2543151152659712TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC850.20015541479266255No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGA700.1648338710057221No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT600.1412861751477618No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT550.12951232721878164No Hit
ATTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT530.12480278804718958No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT470.11067417053241341No Hit
ATTATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT460.10831940094661738No Hit
ATTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC440.10360986177502532No Hit
ATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC430.10125509218922929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAC207.7999335E-570.07429542
AATGACG207.7999335E-570.07429543
ACGTCTA207.7999335E-570.07429547
GAAAGCC150.002200836870.0742942
AAGTATC207.845702E-569.9917533
AGTATCT207.845702E-569.9917534
CCGTATC252.7960687E-669.9917533
CCCGTAT252.7960687E-669.9917532
TCTAATG150.002211165669.9917550
ATCTTAT500.069.991751
GTATCTA150.002211165669.9917535
ACCACCC150.002211165669.9917535
ATCTGCA207.845702E-569.9917552
ATTATAC5750.065.7313841
ACGAATC501.2732926E-1162.9925833
GAATCTC453.7107384E-1062.21489335
CGAATCT453.7107384E-1062.21489334
CTACTGC401.0355507E-861.24278651
AATCTCG401.0355507E-861.24278636
GACGTCT750.060.73105646