##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42467 Sequences flagged as poor quality 0 Sequence length 76 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.72025337320743 27.0 22.0 32.0 13.0 32.0 2 28.445663691807756 32.0 27.0 32.0 13.0 32.0 3 28.996562036404736 32.0 27.0 32.0 22.0 32.0 4 29.887371370711374 32.0 32.0 32.0 27.0 32.0 5 29.610874325947208 32.0 32.0 32.0 22.0 32.0 6 32.822261991664114 37.0 32.0 37.0 22.0 37.0 7 32.13059552122825 37.0 32.0 37.0 13.0 37.0 8 31.24082228553936 37.0 27.0 37.0 13.0 37.0 9 32.814985753644 37.0 32.0 37.0 22.0 37.0 10-11 32.391986719099535 37.0 32.0 37.0 13.0 37.0 12-13 32.48417594838345 37.0 32.0 37.0 17.5 37.0 14-15 32.63006569807145 37.0 32.0 37.0 22.0 37.0 16-17 31.82566463371559 37.0 29.5 37.0 13.0 37.0 18-19 32.20201568276544 37.0 32.0 37.0 13.0 37.0 20-21 32.321802811594885 37.0 32.0 37.0 13.0 37.0 22-23 32.28380860432807 37.0 32.0 37.0 13.0 37.0 24-25 32.1744531047637 37.0 32.0 37.0 13.0 37.0 26-27 32.36772081851791 37.0 32.0 37.0 13.0 37.0 28-29 32.074057503473284 37.0 32.0 37.0 13.0 37.0 30-31 31.849282972661126 37.0 32.0 37.0 13.0 37.0 32-33 31.566169025360868 37.0 29.5 37.0 13.0 37.0 34-35 31.743212376668943 37.0 32.0 37.0 13.0 37.0 36-37 31.60044269668213 37.0 32.0 37.0 13.0 37.0 38-39 31.07144370923305 37.0 27.0 37.0 13.0 37.0 40-41 30.80502507829609 37.0 27.0 37.0 13.0 37.0 42-43 31.057585890220643 37.0 27.0 37.0 13.0 37.0 44-45 31.334224221159957 37.0 27.0 37.0 13.0 37.0 46-47 30.411378246638566 37.0 27.0 37.0 13.0 37.0 48-49 29.798137377257635 37.0 24.5 37.0 10.5 37.0 50-51 30.054206795865024 37.0 27.0 37.0 13.0 37.0 52-53 30.07561165139991 37.0 27.0 37.0 13.0 37.0 54-55 30.092601313961428 37.0 27.0 37.0 13.0 37.0 56-57 29.43312454376339 37.0 22.0 37.0 13.0 37.0 58-59 30.025384416134884 37.0 24.5 37.0 13.0 37.0 60-61 29.709468528504487 37.0 24.5 37.0 13.0 37.0 62-63 30.457272705865734 37.0 27.0 37.0 13.0 37.0 64-65 30.51003131843549 37.0 27.0 37.0 13.0 37.0 66-67 30.55936374125792 37.0 27.0 37.0 13.0 37.0 68-69 30.17176866743589 37.0 27.0 37.0 13.0 37.0 70-71 28.25168954717781 34.5 17.5 37.0 10.5 37.0 72-73 28.16313843690395 34.5 17.5 37.0 8.0 37.0 74-75 29.273341653519203 37.0 22.0 37.0 10.5 37.0 76 29.573833800362635 37.0 22.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.029049775406260636 11101 2 -3.3126381879234756 11101 3 -0.40956418930038296 11101 4 0.494524560566731 11101 5 -1.2216922622283128 11101 6 -1.8412312368068022 11101 7 -2.5338151050356394 11101 8 2.5684494309108388 11101 9 -0.4726787741692391 11101 10-11 -1.7178673535663904 11101 12-13 0.7656115988408843 11101 14-15 0.08627897472434398 11101 16-17 -2.401993933123663 11101 18-19 -0.5422536181098039 11101 20-21 2.215339323054181 11101 22-23 2.173565843886003 11101 24-25 -0.8172316230576122 11101 26-27 -2.04604769549055 11101 28-29 -2.0110303704050736 11101 30-31 3.0084530501734505 11101 32-33 2.0938611720644325 11101 34-35 -1.1551789339596112 11101 36-37 -1.7564564503590567 11101 38-39 -1.4972325816210805 11101 40-41 -1.6073385530033626 11101 42-43 1.5840293488186603 11101 44-45 0.39429355278672773 11101 46-47 -0.8807811954428537 11101 48-49 -1.1432919479883772 11101 50-51 -2.7801744290624057 11101 52-53 2.031984914700999 11101 54-55 -2.0742285616321574 11101 56-57 0.26760918185412663 11101 58-59 -1.906372288453209 11101 60-61 -3.752567496886307 11101 62-63 -0.4674410176311383 11101 64-65 1.0195852656903241 11101 66-67 -0.39326015297769246 11101 68-69 -1.556133825984805 11101 70-71 -4.284601551236452 11101 72-73 -0.9753949019264105 11101 74-75 -3.0339953287615344 11101 76 3.2420736377544586 11102 1 4.620950224593738 11102 2 -1.6126381879234764 11102 3 0.9071024773662835 11102 4 -1.3721421060999361 11102 5 -1.08835892889498 11102 6 2.6587687631932013 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21611 4 0.25875220284315503 21611 5 0.5425353800481112 21611 6 0.49616713717693983 21611 7 0.9478922120375337 21611 8 0.9261730081466126 21611 9 0.8586220388388881 21611 10-11 0.6723765488726343 21611 12-13 1.0674408671335627 21611 14-15 0.7763196251308528 21611 16-17 1.446583302648694 21611 18-19 0.025835812784507084 21611 20-21 -1.253156611905176 21611 22-23 1.315842266650229 21611 24-25 0.28947569401556095 21611 26-27 1.0921636866232731 21611 28-29 2.658685076749393 21611 30-31 2.571461180254758 21611 32-33 1.2645928793815067 21611 34-35 2.5379104969346997 21611 36-37 2.174437858584028 21611 38-39 2.340165792362658 21611 40-41 2.131482585208019 21611 42-43 2.6653301618267946 21611 44-45 0.5528301381525793 21611 46-47 1.5714545769148707 21611 48-49 0.489838133312432 21611 50-51 2.024703619718082 21611 52-53 1.4618629634814866 21611 54-55 2.45321046275809 21611 56-57 1.4363083688459994 21611 58-59 2.4249285245549217 21611 60-61 2.062473153520198 21611 62-63 1.3039004457834942 21611 64-65 2.313284452682197 21611 66-67 1.6382439120629577 21611 68-69 1.7182564179176332 21611 70-71 1.5487317820968798 21611 72-73 0.9372067240898474 21611 74-75 1.973118492376674 21611 76 3.3030492475105575 21612 1 0.6055656092091226 21612 2 1.3924900172047288 21612 3 0.840435810699617 21612 4 1.032986099028271 21612 5 1.2911282505922017 21612 6 1.043384147808581 21612 7 -0.1684304896510227 21612 8 1.5171673796287877 21612 9 1.905526354035885 21612 10-11 1.1026454669464272 21612 12-13 -0.24400378577450255 21612 14-15 0.9805097439551176 21612 16-17 2.5563394002096693 21612 18-19 1.6468489459927653 21612 20-21 -0.3135068307919795 21612 22-23 1.699206869527032 21612 24-25 1.3526401718141834 21612 26-27 -0.09732974677260131 21612 28-29 2.5370465526718533 21612 30-31 0.937940229660633 21612 32-33 1.2733483515516149 21612 34-35 2.3640518352711624 21612 36-37 1.384569190666582 21612 38-39 1.4130238286353283 21612 40-41 0.6843281136633017 21612 42-43 1.2506960154853317 21612 44-45 1.53531919381237 21612 46-47 2.0198598301981754 21612 48-49 1.879143949447517 21612 50-51 2.3608512119632366 21612 52-53 1.2339079916240756 21612 54-55 2.4097457973422003 21612 56-57 1.17145533570028 21612 58-59 1.548755916674999 21612 60-61 0.8435863492675395 21612 62-63 2.4524307772406573 21612 64-65 3.1638160349210906 21612 66-67 1.6932783085607674 21612 68-69 1.3733533535023739 21612 70-71 1.689757423122522 21612 72-73 1.3899897134582062 21612 74-75 1.501261081494878 21612 76 1.998483894164714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 3.0 17 16.0 18 44.0 19 67.0 20 157.0 21 297.0 22 501.0 23 738.0 24 1082.0 25 1407.0 26 1809.0 27 2216.0 28 2604.0 29 3373.0 30 4092.0 31 5154.0 32 5926.0 33 6399.0 34 4712.0 35 1685.0 36 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.614420948523524 43.208684594734606 12.800828898412847 5.3760655583290164 2 23.291025973108532 18.63564650198978 36.06565097605199 22.007676548849695 3 25.897755904584734 16.57051357524666 31.7305201686015 25.8012103515671 4 19.805966986130407 27.717992794405067 22.219605811571338 30.25643440789319 5 21.783973438199073 24.78630466008901 34.1041279110839 19.32559399062802 6 34.39140980055102 37.37961240492618 11.618433136317611 16.610544658205196 7 30.38830150469777 20.667812654531755 12.454376339275202 36.48950950149528 8 27.023335766595242 38.031883580191675 11.222831845903878 23.721948807309204 9 23.729013116066593 13.940235947912496 10.474015117620741 51.85673581840017 10-11 29.611576183579995 24.474585850022958 21.366253399738618 24.547584566658426 12-13 27.504886146890527 13.046600890102903 23.82438128429133 35.62413167871524 14-15 26.30395365813455 13.169048908564296 13.141969058327643 47.38502837497351 16-17 37.45143287729296 22.873054371629735 12.125885982056655 27.54962676902065 18-19 38.41100148350483 16.02538441613488 11.906892410577626 33.65672168978266 20-21 27.82395742576589 14.919820095603646 11.69025360868439 45.565968869946076 22-23 28.236042103280194 24.677985259142392 11.705559610992063 35.38041302658535 24-25 37.98361080368286 23.572420938611156 11.799750394423905 26.644217863282076 26-27 38.30856900652272 25.280806273106176 11.853910094897214 24.556714625473898 28-29 37.162973603032945 16.550498033767397 12.357830786257566 33.9286975769421 30-31 38.713589375279625 23.542986318788707 11.991664115666282 25.751760190265383 32-33 29.1814820919773 23.84792898014929 12.514422963713 34.456165964160405 34-35 36.0632962064662 15.794616996726871 13.21496691548732 34.92711988131961 36-37 29.320413497539267 22.518661548967433 22.748251583582547 25.412673369910756 38-39 26.1567805590223 16.045399957614148 24.324769821272987 33.47304966209057 40-41 25.85183789766171 13.83073916217298 25.186615489674335 35.13080745049097 42-43 35.93185216582483 13.563632510331672 24.151978618440417 26.352536705403086 44-45 25.453903214411866 23.98799010950194 25.918992111150356 24.63911456493583 46-47 34.36576260407712 15.180243307856275 17.791150942729615 32.66284314533699 48-49 33.91519434628975 14.05771495877503 15.739693757361604 36.28739693757362 50-51 25.41424752393626 15.005947263669874 25.184600708960936 34.39520450343292 52-53 22.999917558798245 16.104299897537363 34.01995077082523 26.87583177283916 54-55 22.993134795869103 15.464961552501737 26.527007453986645 35.014896197642514 56-57 32.53031908630637 14.805133639467796 17.414341222182976 35.25020605204286 58-59 24.08534790339484 16.32419956902135 31.98073548979664 27.609717037787174 60-61 33.477375219300825 24.671195940138233 16.286545231899588 25.564883608661354 62-63 23.46555520233596 37.41184227567612 13.211590312363864 25.911012209624058 64-65 23.0720324016295 38.46987072314974 12.557986201050229 25.900110674170534 66-67 23.438199072220783 38.599383050368516 12.194174300044741 25.768243577365958 68-69 23.91386252855158 39.005580803918335 11.932794876021381 25.1477617915087 70-71 27.901664822097157 34.256010549367744 12.067016742411756 25.775307886123343 72-73 44.27555513692985 17.816186686132763 11.733816846021616 26.17444133091577 74-75 46.96470200390892 15.085831351402264 12.13412767560694 25.815338969081875 76 47.85362752254692 14.503025878917747 11.87981256034097 25.763534038194365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 2.0 11 2.5 12 1.0 13 2.0 14 3.0 15 5.0 16 9.5 17 12.0 18 11.5 19 10.5 20 21.0 21 32.0 22 31.0 23 35.5 24 47.5 25 54.0 26 64.0 27 83.5 28 90.5 29 88.0 30 116.0 31 152.0 32 191.0 33 222.0 34 224.5 35 249.0 36 296.0 37 321.0 38 356.0 39 426.5 40 493.5 41 565.5 42 606.0 43 721.5 44 990.0 45 1457.0 46 1771.0 47 2634.5 48 3283.5 49 2001.5 50 934.0 51 766.0 52 602.0 53 555.5 54 505.0 55 542.5 56 596.0 57 713.5 58 815.0 59 859.5 60 962.0 61 1116.5 62 1213.0 63 1265.0 64 1352.0 65 1433.0 66 1466.5 67 1454.0 68 1492.0 69 1469.0 70 1464.5 71 1521.0 72 1462.0 73 1387.0 74 1345.5 75 1320.0 76 1261.5 77 1113.0 78 951.0 79 879.0 80 776.5 81 590.5 82 436.0 83 365.0 84 307.5 85 200.0 86 120.0 87 90.0 88 64.5 89 31.5 90 17.0 91 11.5 92 13.0 93 12.5 94 8.5 95 9.5 96 14.0 97 19.5 98 64.0 99 548.0 100 993.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00235476958579603 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.001177384792898015 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.001177384792898015 44-45 0.00470953917159206 46-47 0.024725080650858315 48-49 0.04003108295853251 50-51 0.024725080650858315 52-53 0.029434619822450374 54-55 0.015306002307674194 56-57 0.00470953917159206 58-59 0.012951232721878165 60-61 0.005886923964490075 62-63 0.001177384792898015 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 42467.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.0528174818094 #Duplication Level Percentage of deduplicated Percentage of total 1 88.25147477961158 62.705159300162485 2 8.484125405978657 12.056420279275674 3 2.1707430237953207 4.627122236089198 4 0.5004308344932724 1.422280829820802 5 0.21541724663617684 0.7653001153837097 6 0.10936567906144362 0.4662443779876139 7 0.04308344932723537 0.21428403230743873 8 0.026512891893683305 0.1507052534909459 9 0.019884668920262475 0.1271575576329856 >10 0.13587857095512693 2.2488049544352084 >50 0.016570557433552065 0.7605905762121177 >100 0.019884668920262475 3.308451268043422 >500 0.0 0.0 >1k 0.006628222973420826 11.147479219158406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 3682 8.670261614900982 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1052 2.4772176042574237 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 439 1.033743848164457 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 319 0.7511714978689336 No Hit ATCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 211 0.4968563826029623 No Hit ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT 194 0.4568252996444298 No Hit ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT 134 0.31553912449666804 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 108 0.2543151152659712 TruSeq Adapter, Index 2 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 85 0.20015541479266255 No Hit ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGA 70 0.1648338710057221 No Hit ATTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT 60 0.1412861751477618 No Hit ATTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT 55 0.12951232721878164 No Hit ATTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT 53 0.12480278804718958 No Hit ATTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT 47 0.11067417053241341 No Hit ATTATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT 46 0.10831940094661738 No Hit ATTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 44 0.10360986177502532 No Hit ATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 43 0.10125509218922929 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.00235476958579603 0.0 3 0.0 0.0 0.0 0.00235476958579603 0.0 4 0.0 0.0 0.0 0.00470953917159206 0.0 5 0.0 0.0 0.0 0.00470953917159206 0.0 6 0.0 0.0 0.0 0.00706430875738809 0.0 7 0.0 0.0 0.0 0.00706430875738809 0.0 8 0.0 0.0 0.0 0.00706430875738809 0.0 9 0.0 0.0 0.0 0.00706430875738809 0.0 10 0.0 0.0 0.0 0.011773847928980148 0.0 11 0.0 0.0 0.0 0.011773847928980148 0.0 12 0.0 0.0 0.0 0.011773847928980148 0.0 13 0.0 0.0 0.0 0.011773847928980148 0.0 14 0.0 0.0 0.0 0.01883815668636824 0.0 15 0.0 0.0 0.0 0.021192926272164268 0.0 16 0.0 0.0 0.0 0.021192926272164268 0.0 17 0.0 0.0 0.0 0.02825723502955236 0.0 18 0.0 0.0 0.0 0.03061200461534839 0.0 19 0.0 0.0 0.0 0.03532154378694045 0.0 20 0.0 0.0 0.0 0.03532154378694045 0.0 21 0.0 0.0 0.0 0.03532154378694045 0.0 22 0.0 0.0 0.0 0.047095391715920594 0.0 23 0.0 0.0 0.0 0.047095391715920594 0.0 24 0.0 0.0 0.0 0.047095391715920594 0.0 25 0.0 0.0 0.0 0.04945016130171663 0.0 26 0.0 0.0 0.0 0.05180493088751266 0.0 27 0.0 0.0 0.0 0.09654555301763722 0.0 28 0.0 0.0 0.0 0.12009324887559752 0.0 29 0.0 0.0 0.0 0.16483387100572208 0.0 30 0.0 0.0 0.0 0.24018649775119505 0.0 31 0.0 0.0 0.0 0.29434619822450375 0.0 32 0.0 0.0 0.0 0.37440836414156875 0.0 33 0.0 0.0 0.0 0.548661313490475 0.0 34 0.0 0.0 0.0 0.8123955070996303 0.0 35 0.0 0.0 0.0 1.172675253726423 0.0 36 0.0 0.0 0.0 1.5659217745543599 0.0 37 0.0 0.0 0.0 2.220547719405656 0.0 38 0.0 0.0 0.0 2.8539807379847884 0.0 39 0.0 0.0 0.0 3.4308992865048156 0.0 40 0.0 0.0 0.0 4.0737513834271315 0.0 41 0.0 0.0 0.0 4.636541314432383 0.0 42 0.0 0.0 0.0 5.182847858337062 0.0 43 0.0 0.0 0.0 5.792733181058233 0.0 44 0.0 0.0 0.0 6.388489886264629 0.0 45 0.0 0.0 0.0 7.0478253702875175 0.0 46 0.0 0.0 0.0 7.68125838886665 0.0 47 0.0 0.0 0.0 8.279369863658841 0.0 48 0.0 0.0 0.0 8.806838250877151 0.0 49 0.0 0.0 0.0 9.308404172651706 0.0 50 0.0 0.0 0.0 9.819389172769444 0.0 51 0.0 0.0 0.0 10.269150163656485 0.0 52 0.0 0.0 0.0 10.839004403419125 0.0 53 0.0 0.0 0.0 11.314667859749923 0.0 54 0.0 0.0 0.0 11.787976546494926 0.0 55 0.0 0.0 0.0 12.134127675606942 0.0 56 0.0 0.0 0.0 12.55563143146443 0.0 57 0.0 0.0 0.0 12.904137330162243 0.0 58 0.0 0.0 0.0 13.299738620575976 0.0 59 0.0 0.0 0.0 13.676501754303342 0.0 60 0.0 0.0 0.0 14.03913627051593 0.0 61 0.0 0.0 0.0 14.41825417382909 0.0 62 0.0 0.0 0.0 14.778533920455883 0.0 63 0.0 0.0 0.0 15.127039819153696 0.0 64 0.0 0.0 0.0 15.475545717851508 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATGAC 20 7.7999335E-5 70.074295 42 AATGACG 20 7.7999335E-5 70.074295 43 ACGTCTA 20 7.7999335E-5 70.074295 47 GAAAGCC 15 0.0022008368 70.07429 42 AAGTATC 20 7.845702E-5 69.99175 33 AGTATCT 20 7.845702E-5 69.99175 34 CCGTATC 25 2.7960687E-6 69.99175 33 CCCGTAT 25 2.7960687E-6 69.99175 32 TCTAATG 15 0.0022111656 69.99175 50 ATCTTAT 50 0.0 69.99175 1 GTATCTA 15 0.0022111656 69.99175 35 ACCACCC 15 0.0022111656 69.99175 35 ATCTGCA 20 7.845702E-5 69.99175 52 ATTATAC 575 0.0 65.731384 1 ACGAATC 50 1.2732926E-11 62.99258 33 GAATCTC 45 3.7107384E-10 62.214893 35 CGAATCT 45 3.7107384E-10 62.214893 34 CTACTGC 40 1.0355507E-8 61.242786 51 AATCTCG 40 1.0355507E-8 61.242786 36 GACGTCT 75 0.0 60.731056 46 >>END_MODULE