FastQCFastQC Report
Wed 25 May 2016
SRR1779596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210252
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92084.379506496965546No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG5470.26016399368376997No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATC252.3865303E-456.00000421
GCACTTA200.00694417852.513
TTTCGTT350.001253430440.018
TCGTGTA459.1762675E-538.8888913
CGTATCA1300.037.69230756
GCCGTAT1300.037.69230754
AGTCATG400.002414954835.036
TTAATAT400.002414954835.058
TAGATCT1700.035.039
ATTTTCG652.0959042E-532.30769316
AGATCTC1750.032.040
CATTAAA1650.031.81818261
CCGTATC1550.031.61290455
GTATCAT1450.031.3793157
GCCTACT450.00430053531.11111335
TCGGTGG2050.030.73170945
TCATTAA1650.029.6969760
TAGTGTA2150.029.30232634
TTTTCGT604.9706176E-429.16666617
GTAGATC2050.029.02439138
Produced by FastQC (version 0.11.5)