FastQCFastQC Report
Wed 25 May 2016
SRR1779596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210252
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT42512.021859482906227No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT11440.544108973993113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10330.49131518368434074No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG6490.3086772063999391No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2740.1303198067081407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT150.002223777669.9888222
ATCTAGT150.002223777669.9888237
ATTATAC7800.058.7726631
GTATGCC9750.054.9535142
CGTATGC10200.054.5760741
AGTCTTC452.6726411E-854.4875848
CCGTCTT9250.054.1509847
GCCGTCT9700.053.44437446
GTCTTCT9900.053.38801249
TCGTATG10900.053.29424340
CTTGAAA10600.053.1771857
TATGCCG10150.053.13287443
TCTTCTG9700.052.6845350
ACACAGT200.00694856452.491626
ACGGGTA200.00694856452.4916221
CTCGTAT10600.052.49161539
TTGAAAA10950.052.11692458
AGGGGGG4850.051.95046670
TCTCGTA10650.051.9165938
CGTCTTC10150.051.76511848