FastQCFastQC Report
Wed 25 May 2016
SRR1779595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92557
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1102511.911578810894909No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG8290.8956642933543655No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330.14369523644889096No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.10696111585293386No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAA150.00221842970.017
TCGTTCC150.00221842970.04
GCCCAAT150.00221842970.026
GACATAC150.00221842970.040
CTTTATA150.00221842970.02
GTTGGAT150.00221842970.064
GCACTCC150.00221842970.036
GCTAAAG308.319625E-658.3333321
CGTGCTA200.00693195752.50000423
AGCTATA200.00693195752.5000042
ATTTGCA200.00693195752.50000435
TAGCTGC200.00693195752.50000415
TGCACTC200.00693195752.50000435
CTTGTGC350.001249796640.01
GCCGTAT2200.036.5909154
CGTATCA2350.035.74468256
GCGAGAC400.00240799635.0000046
GTGTAGG400.00240799635.00000436
TAGATCT2550.034.31372539
GTATCAT2350.034.2553257
Produced by FastQC (version 0.11.5)