FastQCFastQC Report
Wed 25 May 2016
SRR1779595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92557
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT56276.079496958630898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14941.61414047559882No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6630.7163153516211632No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG6150.6644554166621649No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC3890.4202815562302149No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT2230.24093261449701264No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT1620.17502728048661906No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1070.11560443834610025Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1000.10804153116457965No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT207.8239755E-570.1191144
CGAATGC207.8239755E-570.1191141
GAGTGTA207.887184E-570.005438
TCGAAAG150.002217745970.005440
GTCTCCA150.002222507769.967581
TAGCACG150.002222507769.9675861
CCGAACC150.002222507769.9675833
GCAGCTA150.002222507769.9675869
AGTAGTG150.002222507769.967585
GTAACGG150.002222507769.9675825
ATTATAC9050.064.555731
ATCGTAT601.8189894E-1258.33783339
TCTAGCA252.3786136E-456.00432659
CAAGAAA252.3786136E-456.00432657
ATATCGT509.695214E-1055.97406837
AGGGGGG5200.055.8395270
TATGCAG452.6318958E-854.5370943
CCGTCTT10150.053.53922747
CGTCTTA200.00688546352.58933348
AATGCCG200.00688546352.58933343