FastQCFastQC Report
Wed 25 May 2016
SRR1779594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293158
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123954.228095429768248No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG8730.2977916345451941No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA150.00222324770.024
ACGCTTG150.00222324770.051
CGTATCT200.006946896752.50000456
GCCGTAT2000.045.50000454
CCGAAAT404.586392E-543.75000447
CGTATCA2200.041.3636456
CCGTATC2500.039.255
GTAGATC2500.036.39999838
CTCGTAA400.002416503835.00000422
TCGTAAG400.002416503835.00000423
GCATGTA400.002416503835.0000042
ACTGGTC501.7073826E-435.00000412
TCTCGGT2400.035.043
GTATCAT2700.035.057
CGCCGTA2550.034.31372553
TAGATCT2600.033.65384739
AGATCTC2650.033.01886740
TGGTCGC2900.032.5862149
TCGCCGT2700.032.40740652
GTGGTCG2950.032.03389748
Produced by FastQC (version 0.11.5)