FastQCFastQC Report
Wed 25 May 2016
SRR1779594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293158
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT60492.063392436842931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15140.516445056931757No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT13630.4649369964319582No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG9600.3274684641046808No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4270.14565524392989446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC11350.059.1982461
CACGTAT252.389844E-455.9916839
CTTGAAA14500.053.1136757
CCGTCTT13200.053.0676847
TCGTATG14850.053.02242340
TATGCCG14150.052.9611343
CGTATGC14700.052.85830341
CTCGTAT14450.052.31056239
GTCTTCT14000.052.0188949
ATGCCGT14200.051.79704344
TTGAAAA15150.051.75914458
TCTCGTA14300.051.63567738
TCTTCTG14250.051.59768350
GTATGCC14550.051.48757642
GCCGTCT14050.051.3528946
AGGGGGG7300.051.29374770
ATCTCGT14100.051.12714837
TGCCGTC14400.050.8344145
CGTCTTC14000.050.78576748
TCTGCTT14400.050.0961853
Produced by FastQC (version 0.11.5)