FastQCFastQC Report
Wed 25 May 2016
SRR1779593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481039
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236804.922677787040136No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG16530.34363118167134055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG305.8759743E-446.66666817
CGTTACA305.8759743E-446.66666814
CGTATCA4000.040.2556
TCATTAA3900.039.4871860
GTATCAT4000.039.37557
GTTATAC459.193922E-538.8888851
ATCATTA4150.037.95180559
TAGATCT4250.037.8823539
TATCATT4350.036.20689458
CATTAAA4350.035.40229861
CCGTATC4450.035.39325755
GTACACT400.002418039435.06
TTCGTTA400.002418039435.012
GTAGCAT400.002418039435.03
GCGCTAG501.7090079E-435.01
ATTAAAA4600.034.23913262
GCCGTAT4650.033.87096854
TTAATAT552.990886E-431.8181844
TGTAGCA552.990886E-431.8181842
TGTTACG450.00430599631.11110918
Produced by FastQC (version 0.11.5)