FastQCFastQC Report
Wed 25 May 2016
SRR1779593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481039
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT111982.327877781219402No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT33680.7001511311972626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29500.6132558898550845No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG18350.3814659518251119No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC8280.17212741586441016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC17750.064.279111
AGGGGGG12900.060.50144670
CTTAATA608.367351E-1152.4978942
TTACGAT200.00695069452.4978943
CTTTATA407.477247E-752.4978942
TCGTATG27600.051.48344440
CTCGTAT26850.051.35734639
CTTGAAA26250.050.93658457
CGTATGC26550.050.62470241
GTATGCC25800.050.6092742
TCTCGTA27550.050.43354838
CCGTCTT24750.050.21044547
TGAAAAA28300.049.96785759
TTGAAAA27250.049.9664958
GTCTTCT26250.049.8698549
TCTTCTG26150.049.79285450
TCTGCTT25650.049.67695253
TATGCCG26250.049.60832243
GAAAAAA30100.049.41662660
TAATCTC27200.049.2811135
Produced by FastQC (version 0.11.5)