FastQCFastQC Report
Wed 25 May 2016
SRR1779592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351708
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176595.020926450350859No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG10340.29399388128788656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA200.00694804652.562
GGCTTAA200.00694804652.562
CCGTATC1750.042.055
CGTATCA1900.038.6842156
GCCGTAT1950.037.69230754
ATCATTA2200.033.4090959
GTATCAT2300.031.95652457
TGAAACG450.00430443731.1111095
TAGATCT2400.030.62539
TCATTAA2450.030.060
CATTAAA2600.029.61538361
CGCCGTA2850.029.47368453
TACTGCG604.977345E-429.1666662
TCGGTGG2900.028.96551745
TCTCGGT2550.028.8235343
ACTCGAC500.007203673527.99999863
GTAGATC2650.027.7358538
CTAAAAG657.9482986E-426.9230773
TATCATT2800.026.2558
GTATAAG808.653892E-526.251
Produced by FastQC (version 0.11.5)