FastQCFastQC Report
Wed 25 May 2016
SRR1779592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351708
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT83372.370432290422737No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT27650.7861635220125787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19340.5498879752521979No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG12320.35029058196003504No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6670.18964595630466183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC14750.063.3363721
AGGGGGG11200.056.23263570
CCGTCTT20850.051.10260447
GCCGTCT20850.051.10260446
TCGTATG22450.050.97871440
GTCTTCT20850.050.90550249
CGTATGC22150.050.89359741
TATGCCG21800.050.7832643
GTATGCC21650.050.64964742
CTTGAAA21750.050.527557
CTCGTAT22450.050.50383839
TTCTGCT20650.050.04147752
TGAAAAA23850.049.60046459
TCTTCTG21350.049.54926750
TCTCGTA23350.049.4564338
CGTCTTC21900.049.29264548
GCTTGAA22050.049.21914756
TAAGATC23250.049.2106133
TCTGCTT21400.048.949453
TTGAAAA22900.048.60143358