FastQCFastQC Report
Wed 25 May 2016
SRR1779591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160434
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1829211.401573232606555No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG10230.637645386888066No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2200.13712804019098196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGG252.3851857E-455.9982532
GTCCTAT252.3851857E-455.9982531
TTCCATC252.3851857E-455.99825325
CAATAAA200.0069334952.51473261
AAAAGCC200.006942047352.49836330
AATTGGA200.006942047352.49836333
AAAAAGG1150.045.65075370
CGTATCA1700.045.292756
TCATTAA1600.043.76227660
TAGATCT2000.043.74863439
ATCATTA1650.042.4361559
CATTAAA1750.042.01178461
GTAGATC2150.040.69640738
GCCGTAT1900.040.52505554
TATCATT1650.040.31434258
GTATCAT1750.040.01122357
CCGTATC1950.039.4859555
TGTAGAT2150.039.0685537
ATTAAAA1900.038.6950762
TAGTGTA2200.038.1806334