FastQCFastQC Report
Wed 25 May 2016
SRR1779591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160434
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT66604.151239762145181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19591.2210628669733348No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT17541.0932844658862835No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG9300.5796776244436964No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT5100.31788772953364No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT4960.30916139970330475No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4550.2836057194858945No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT2250.14024458655895883No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA1820.1134422877943578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA150.00221685670.0361948
CGTATGG207.8998404E-570.0143441
GTATTAT150.002219600870.0143449
CCTAAGC207.8998404E-570.0143441
GTAAGAC301.01723344E-770.0143442
TAAGACG353.6798156E-970.0143443
GCTTTAA301.01723344E-770.0143456
ATGGGTT150.002222348269.992527
ATCGTAA150.002222348269.9925239
ATTATAC13200.068.136661
GTATTCT550.063.64940349
TAGTATG2200.063.62956240
TCTAGTA2450.062.85042638
AGTATGC1850.062.4452241
ATCTAGT2500.061.59341437
CGTAAGC800.061.26255441
CTAGTAA401.0602889E-861.24345439
TTTAAAA352.9555304E-760.01229558
CTTTAAA352.9555304E-760.01229557
AATAAGC352.9555304E-760.01229551