FastQCFastQC Report
Wed 25 May 2016
SRR1779590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219032
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213989.769348770955842No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG19090.8715621461704227No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2610.11916067058694621No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCG150.00222249470.02
CTTGTCT200.006944562352.50000426
TTGTCAC200.006944562352.50000425
TGTACGT200.006944562352.50000468
TCTGGAA200.006944562352.50000450
CTCAGTT200.006944562352.5000044
TATGCAA305.8689277E-446.66666410
TAGTGTC305.8689277E-446.66666419
GCTTTAA305.8689277E-446.6666641
TCAAGTG602.5869667E-740.83333232
AGCTGTA350.001253544840.043
TGGTCGC4150.039.63855749
GCCGTAT3800.039.60526754
CGGTGGT4450.039.32584446
AGATCTC4050.038.02469340
TAGATCT4050.038.02469339
TCTCGGT4200.037.543
ATCTCGG4150.037.10843742
CCGTATC4100.036.70731755
GTAGATC4200.036.66666438
Produced by FastQC (version 0.11.5)