FastQCFastQC Report
Wed 25 May 2016
SRR1779590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219032
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT94934.334069907593411No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT36081.6472478907191643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32481.4828883450819972No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG15470.7062894919463822No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6890.3145659081778005No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4710.21503707220862706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3710.1693816428649695No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT2700.12326965922787537No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2540.11596479053289016No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT2310.10546404178384894Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTC150.00221961970.0228442
ATCGTAT453.8198777E-1062.22831339
ATTATAC15400.062.0515251
CTTAAGC401.0590156E-861.2699951
GTCTTAA401.0590156E-861.2699949
AGGGGGG10550.059.7078570
GTCTTCA1450.057.94993649
AATCTCG1000.055.9926936
AGCTTGA2200.054.1085655
GCACAAC200.00693560552.5171343
GGATCCA200.006948146552.4931537
GATCTCA200.006948146552.49315311
CGCGTGT200.006948146552.49315320
GCGAGTT200.006948146552.49315333
CCGTCTT18200.051.36290447
GCCGTCT18150.051.11860746
GTATGAC1650.050.925742
CGTCTTC18500.050.908548
CGTATGC21600.050.57205241
TATGCCG20850.050.54406443