FastQCFastQC Report
Wed 25 May 2016
SRR1779589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628598
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167582.6659327582970356No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG11100.17658344442712193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2500.050.39908656
GTATCAT2800.043.74920757
CCGTATC2950.042.71108655
GCCGTAT2900.042.2406154
TAGATCT3250.040.92558739
ATCATTA3350.035.52174459
GTAGATC4200.033.33537738
TATCATT3700.032.1615858
TCATTAA3700.032.1615860
AGATCTC4350.030.57415640
CATTAAA3950.030.12603261
TCGGTGG4900.027.8588545
TAGTGTA4900.027.14452234
TGGTCGC5150.026.50437249
CGCCGTA4500.026.44396453
TGTAGAT5100.026.08003237
TCTCGGT5000.025.9015943
AGCTTAA1251.09874236E-725.19954312
GTGTAGA5350.024.86133836
GTCCTAT851.3082597E-424.7054331
Produced by FastQC (version 0.11.5)