FastQCFastQC Report
Wed 25 May 2016
SRR1779589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628598
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT82011.304649394366511No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20840.33153143980731725No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT16060.2554891997747368No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG10910.17356084492791896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC13850.055.5832821
CGTATGA1000.052.53427541
TCGTATG16650.051.70019540
CTCGTAT16750.050.9737239
CGTATGC16450.050.03264241
TATGCCG16150.049.90157743
GTATGCC16350.049.71978442
CCGTCTT14350.049.08374847
TCTCGTA17650.048.5727538
TTGAAAA16100.048.25779758
CTTGAAA15750.048.2191557
TCTTCTG14250.047.91837750
GTCTTCT14350.047.096449
TGAAAAA18850.046.7874759
CGTCTTA900.046.72317548
GTTACGT305.8838155E-446.6562731
ATGCCGT17300.046.58442344
GCCGTCT16100.045.92144846
CGTCTTC15450.045.58911548
TTCTGCT14200.045.37088452
Produced by FastQC (version 0.11.5)