FastQCFastQC Report
Wed 25 May 2016
SRR1779588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66006
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1988130.119989091900734No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG15322.321001121110202No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2170.32875799169772446No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1550.23482713692694604No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1490.22573705420719328No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG900.13635124079629124No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC830.12574614428991304No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.12574614428991304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA150.002215598770.0000116
GCCGTAT2250.059.1111154
GTATCAT2350.058.08510657
CCGTATC2300.057.82608455
CGCCGTA2300.057.82608453
ATCATTA2550.057.6470659
TCATTAA2550.057.6470660
CATTAAA2500.057.39999861
CGTATCA2400.056.87500456
TATCATT2400.056.87500458
TGGTCGC2500.056.049
GAGGACT252.374505E-455.9999964
ACTAGTG252.374505E-455.99999632
ATTAAAA2650.055.471762
AGTGTAG2600.055.19230735
TGTAGAT2600.055.19230737
GTAGATC2550.054.90196238
GTGTAGA2650.054.15094436
TTAAAAA2650.054.15094463
TAGATCT2600.053.84615339