FastQCFastQC Report
Wed 25 May 2016
SRR1779588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66006
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1010015.301639244917128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25753.9011605005605547No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT14412.1831348665272854No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG12911.9558827985334666No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5600.8484077205102567No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT4410.6681210799018271No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT3090.46813926006726664No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT2010.30451777111171713No Hit
ATTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT1850.2802775505257098No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT1640.24846226100657515TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1560.2363421507135715No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1550.23482713692694604No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGA1520.2302820955670697No Hit
ATTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT1000.15150137866254582No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTAAGCCGT1000.15150137866254582No Hit
ATTATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT950.14392630972941853No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCACGTATGCCGT940.14241129594279306No Hit
ATTATACACATCACCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT880.13332121322304033No Hit
ATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC840.12726115807653848TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC730.11059600642365847No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT700.10605096506378209No Hit
ATTATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT700.10605096506378209No Hit
TATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG680.10302093749053116No Hit
ATTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC670.1015059237039057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGA207.799729E-570.1328744
CAGTATT150.002198975370.1328747
ACGTATT207.799729E-570.1328747
GGCTGTT150.002198975370.1328742
TAAGACG207.799729E-570.1328743
AGTATTC207.799729E-570.1328748
CCGTAAT150.002198975370.1328747
GCAGTAT207.799729E-570.1328746
CGTATTA150.002198975370.1328748
GTAAGAC150.002198975370.1328742
CGTAATC150.002198975370.1328748
TGACGTA207.799729E-570.1328745
CGACTAA150.002198975370.1328748
CCTTGGT150.002212263670.02653552
TTCTTGA207.858656E-570.02653555
GTCTTAA150.002212263670.02653549
TTAAGCA150.002212263670.02653552
TCTTGAA150.002212263670.02653556
CGTAAGA150.002212263670.02653541
ATTCTGC207.858656E-570.02653551
Produced by FastQC (version 0.11.5)