FastQCFastQC Report
Wed 25 May 2016
SRR1779587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289465
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210307.2651270447204315No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG14050.4853781977095677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA150.002221847770.0108963
GCCGTAT2400.056.8740254
TCCGGTT252.3862053E-456.0087161
TAGATCT2450.054.2847839
GTAGATC2500.053.19908538
CCGGTTT200.006942538552.50816362
CCGTATC2700.051.85095255
CGCCGTA2750.050.9082153
CGTATCA2800.049.99913856
TCGGTGG3000.048.9991545
AGATCTC2900.048.2750340
ATCTCGG2850.047.89390642
TGGTCGC2950.047.4568149
TCTCGGT3050.045.90084543
GTATCAT3250.044.16071357
CGGTGGT3250.044.15308446
TCATTAA3300.043.49160860
CATTAAA3500.042.0065361
TGTAGAT3600.041.80483237
GTGTAGA3700.041.62090336
Produced by FastQC (version 0.11.5)