FastQCFastQC Report
Wed 25 May 2016
SRR1779587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289465
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT82122.8369578360078074No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT43891.516245487364621TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24320.8401706596652445No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG17120.5914359248959288TruSeq Adapter, Index 5 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5090.1758416388855302TruSeq Adapter, Index 5 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4210.1454407268581694RNA PCR Primer, Index 5 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3590.12402190247525607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC13800.061.117461
GTCTTAA308.360412E-658.3379449
ATCGTAT1000.056.00442539
AGGGGGG11300.054.82733570
CAACGGT200.006935151352.52229744
AACGGTC200.006935151352.52229745
CGTAATC407.442886E-752.52229748
CGTATGA1200.052.51321841
ATCTTGA200.00694463852.50414755
CTTTATA407.469571E-752.495082
TCTTCTG19350.051.91624550
CGTATGC22750.051.5514441
TCGTATG22700.051.5018640
CGTCTTC20600.051.16249548
CCGTCTT20700.051.08448847
CTTGAAA21550.051.00170557
CTTCTGC19950.050.88121451
CTGCTTG18800.050.8284854
ATCTCGT22200.050.76977537
TATGCCG23200.050.71118543
Produced by FastQC (version 0.11.5)