FastQCFastQC Report
Wed 25 May 2016
SRR1700333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944921
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA250100.36011928717403696No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA118670.1708730740061694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.1242922705672246No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT78940.11366579979815465No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT78380.11285945513275096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG79550.027.519732
GTACCTG83400.026.770971
ACCTGGG86300.024.818693
TATAACG5450.023.1186772
TAACGCA6000.020.9973494
CCTGGGG62800.020.3477484
ATAACGC6450.019.1847293
GTACACG5800.015.91015151
GTACCGG6100.015.4966541
TACACGG6000.014.9996192
CTGGGGG32550.014.8591265
GTATAAC15150.014.8561341
TACCGGG5850.013.8458012
GTACCCG7350.013.1673861
GTACATG168900.013.08581
TCACGCA5850.013.0752724
ACGTCCG3450.013.04183243
TACATGG168150.012.7249212
AGAGTAC224100.012.47780611
TATCACG6900.012.3909892
Produced by FastQC (version 0.11.5)