FastQCFastQC Report
Wed 25 May 2016
SRR1700332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7147706
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96190.13457464534775213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG92650.030.0757581
TACCTGG92150.029.8111922
ACCTGGG101700.026.7015363
GGTATCA78400.024.5753141
TATAACG6700.024.51362
ATAACGC7300.023.11473
CCTGGGG69850.022.5778874
TAACGCA7500.022.1974014
GTATCAA149650.020.906411
GTACCGG6850.020.3724141
TACCGGG6500.019.3835662
GTACCCG8150.018.7798731
TACCCGG8700.017.8438632
ATCAACG183400.016.7817273
TCAACGC184750.016.670584
CAACGCA188800.016.347925
TATCAAC190250.016.2487932
CTGGGGG36550.015.9412645
AACGCAG198150.015.8150476
GTACACG6100.014.7594961
Produced by FastQC (version 0.11.5)