FastQCFastQC Report
Wed 25 May 2016
SRR1700331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9827851
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142600.1450978448899968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100760.1025249568801969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG97050.025.1292882
GTACCTG100700.024.7870771
GGTATCA112750.023.8946591
ACCTGGG101350.023.4190083
TATAACG7050.023.2959382
TAACGCA7700.021.9129834
GTATCAA211500.021.4005491
ATAACGC8300.019.5161513
CCTGGGG78450.018.6689114
GTACCGG8650.017.6935941
ATCAACG253800.017.400773
TCAACGC255150.017.3171674
CAACGCA261850.016.8989875
TATCAAC264200.016.8352782
AACGCAG271750.016.33316
TACCCGG9050.014.9158882
GTACCCG10050.014.7809091
ACGCAGA314950.014.0712757
TACCGGG9700.013.91636852
CGCAGAG319150.013.8226598
Produced by FastQC (version 0.11.5)