FastQCFastQC Report
Wed 25 May 2016
SRR1700330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14767179
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162370.10995329575134154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195800.020.5884781
GTATCAA339650.018.8187561
GTACCTG96150.018.1024531
CGCGTAT1551.0966687E-817.4146612
TACCTGG97900.016.8396222
TAACGCA8650.015.6214524
ATCAACG402250.015.5197053
TCAACGC404100.015.4598014
ACCTGGG104600.015.2866733
CAACGCA410100.015.2317595
AACGCAG420450.014.9318086
TATCAAC423500.014.8160222
ATAACGC9700.014.3948193
GTACATG324800.013.5220451
CGTCGAC2002.5048575E-713.49640742
TATAACG9700.013.2344872
TACATGG318900.013.0159182
ACGCAGA481000.013.0053737
AGAGTAC446050.012.92499711
GAGTACT280200.012.91675312
Produced by FastQC (version 0.11.5)