FastQCFastQC Report
Wed 25 May 2016
SRR1700329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8921908
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120240.1347693789265704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90400.10132361822157324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG96950.025.2750741
TACCTGG95050.025.2599832
ACCTGGG101400.023.188153
GGTATCA112250.020.5246281
GTATCAA195150.018.9099711
CCTGGGG78450.018.1437784
TATAACG5750.018.018472
ATAACGC6650.016.9337043
TACCCGG9000.015.7661622
GTACCCG9200.015.6820521
GTACACG7800.015.6066571
ATCAACG231200.015.5568153
TCAACGC232750.015.4435374
TACACGG7900.015.3955292
CAACGCA237350.015.2012065
TAACGCA7050.015.014554
TATCAAC243400.014.9074352
AACGCAG244450.014.8885156
GTACATG209550.013.3504171
GTACCGG7350.013.1883921
Produced by FastQC (version 0.11.5)