FastQCFastQC Report
Wed 25 May 2016
SRR1700328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14829543
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA482730.32551913433879925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA228490.15407757339521522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175030.11802791225596096No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT157530.10622714401920545No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT150800.10168890572015606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG96350.018.4104141
TACCTGG97200.017.5158942
ACCTGGG109150.015.2466353
TAACGCA8000.014.3560024
ATAACGC8550.013.69593
TATAACG8300.013.29519752
AGAGTAC460900.012.88483311
GAGTACT305150.012.77512912
GTACATG307250.012.7276461
GTATTAG41950.012.4651211
AGTACTT315050.012.30942713
GTACCCG10100.012.2738791
TACATGG304050.012.2212522
GTATCAA580900.012.1484871
CGCGTAT1506.4697594E-411.9971712
CGCGGTA6550.011.67746443
ACATGGG303850.011.6579663
TCTATAC36400.011.5689393
TATACTG51750.011.5625295
GTATAAC29450.011.4035861
Produced by FastQC (version 0.11.5)