FastQCFastQC Report
Wed 25 May 2016
SRR1700327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11931194
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA512870.4298563915732156No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA245450.2057212379582463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175780.14732808803544725No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT169360.14194723512164834No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT166460.13951663178052423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134520.11274646946483312No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC132770.11127972606932719No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA119900.10049287606923499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126350.025.2955381
TACCTGG125650.024.8227162
ACCTGGG132000.023.1328723
TATAACG9150.018.2126272
ATAACGC9800.017.6932833
CCTGGGG103750.017.1467864
TAACGCA10200.016.116344
GTACCCG12700.015.0854451
GTACCGG11500.014.6996291
GTACACG9150.014.2872891
TACCCGG12850.013.3190252
GTACATG288550.013.122941
TACATGG287750.012.60788252
GAGTACT259800.012.45977312
AGAGTAC414500.012.44970911
GTATAAC24700.012.2278691
AGTACTT265200.012.19758513
TATACTG38000.011.9578885
ACATGGG294400.011.7794953
GTATTAG31750.011.7134051
Produced by FastQC (version 0.11.5)