FastQCFastQC Report
Wed 25 May 2016
SRR1700326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11895594
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143730.12082624877748854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144750.023.0130791
GTATCAA258600.019.8886811
GTACCTG87100.019.5882931
TACCTGG88100.019.0172822
TAACGCA7000.017.6929234
ACCTGGG94350.017.3511183
TATAACG7050.017.2486882
ATAACGC10150.016.6391143
ATCAACG323900.015.6286093
TCAACGC328350.015.3893684
CAACGCA334550.015.0953375
TATCAAC340200.014.9664112
AACGCAG345000.014.7228666
CGCGGTA6050.014.50144743
GTACATG294800.013.746151
ACCGTCG1709.440861E-613.232518
GTATTAG35000.013.1365391
TACATGG291900.013.1149382
TATACTG42150.013.0754315
ACGCAGA393050.012.8716087
Produced by FastQC (version 0.11.5)