FastQCFastQC Report
Wed 25 May 2016
SRR1700325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10288036
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114540.11133320295535513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG108450.026.3962692
GTACCTG111200.026.193811
ACCTGGG118700.023.5650223
CCTGGGG84350.018.6633264
TACCCGG8600.018.0707912
ATAACGC8500.017.7522263
GTACCGG7750.017.7447171
GTACCCG9400.017.268141
GGTATCA152050.017.1845421
GTACACG8150.016.8738121
GTATCAA242750.016.726131
TAACGCA8900.016.7013234
TATAACG8900.016.1963272
TACCGGG7900.015.3954782
GTCGTAC3000.014.2781931
CGCTACG5950.013.9888928
GTATTAG30450.013.6969981
ATCAACG290150.013.6378573
TCAACGC290950.013.623584
TACACGG8550.013.4347812
Produced by FastQC (version 0.11.5)