FastQCFastQC Report
Wed 25 May 2016
SRR1700324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8432552
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120000.14230567448620535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92310.10946864009851347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG115900.029.0559641
TACCTGG114050.028.9508422
TATAACG8350.027.7826232
ACCTGGG124450.026.1852933
TAACGCA8900.024.7981854
ATAACGC9500.024.180233
GTACCGG8550.023.1998251
CCTGGGG81100.020.9101894
GGTATCA113300.020.4121
GTACCCG9700.019.9845731
TACCGGG8350.018.8813932
GTATCAA194000.018.6600151
TACCCGG10300.018.3681132
GTATAAC18800.016.5458621
CGCGGTA4150.015.17772943
ATCAACG240750.014.6771073
TCAACGC242950.014.5442024
TATCAAC249050.014.3067972
CAACGCA248100.014.2620955
ACAACGC4150.014.1094173
Produced by FastQC (version 0.11.5)