FastQCFastQC Report
Wed 25 May 2016
SRR1700323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11847392
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA196130.1655469828296388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163430.13794597156910143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128080.10810818110855115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG121950.024.4522482
GTACCTG125550.024.0762141
ACCTGGG132950.021.8184363
ATAACGC9300.018.6467823
TATAACG9400.018.2095132
TAACGCA9250.017.5302014
CCTGGGG105550.016.9630934
GTATCAA351350.015.3011671
GTACCCG13000.014.3916591
TACCCGG12100.014.1462342
GGTATCA235700.013.9913911
GTATTAG29700.013.5095121
GTACATG279500.013.2827461
ATCAACG418050.012.660063
TCTATAC28000.012.6280923
TCAACGC419800.012.6180134
TACATGG282050.012.6086522
GTACCGG11650.012.5766091
TATACTG39000.012.5163515
TATCAAC430250.012.3957692
Produced by FastQC (version 0.11.5)