FastQCFastQC Report
Wed 25 May 2016
SRR1700322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10392854
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA236980.22802206208227307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154270.1484385328611371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115630.11125914017458534No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA113220.10894023912969432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG89900.023.725452
GTACCTG92500.023.5704211
TATAACG7200.021.8972592
TAACGCA8300.020.894234
ACCTGGG100550.020.6299213
ATAACGC8100.020.5762733
GTACCCG8550.016.8770471
CGCGTAT2009.331416E-1015.74691112
CCTGGGG75800.015.5398294
GTATCAA354650.013.9927361
TACCCGG10200.013.91122
GTACCGG7400.013.4061341
GTACATG215350.013.024411
TAGACCG1751.272179E-512.8548085
GGTATCA249600.012.7457381
GTACACG8350.012.6909461
GAGTACT235300.012.51454512
AGAGTAC345800.012.4774911
TACATGG215650.012.4285862
TATACTG37250.012.3803015
Produced by FastQC (version 0.11.5)